The Florida State Collection of Arthropods is worldwide in scope. Earlier accumulations, primarily from Florida and the southeastern United States, still form a large portion of the collection; however, most insect groups have worldwide representation, with particular strengths for circum-Caribbean and South American regions. In recent years much new material has been obtained, through surveys or exchanges, from the Neotropics, parts of Africa (especially South Africa) and Asia (especially Indonesia and Taiwan).

Among the 22,400 drawers of pinned insects, 352,000+ slides, and 294,200+ vials of the Museum of Entomology, are an estimated 9 million prepared specimens including over 2,500 primary and at least 30,000 secondary types. Millions more specimens are in the estimated 20,000 bulk alcohol containers and various dry samples from around the world. These, together with the other holdings, place the FSCA among the top 10 North American entomological collections.