The Arachnids collection of the Museo Nacional de Historia Natural “Prof. Eugenio de Jesús Marcano” (MNHNSD-ARACH) houses more than 4000 lots containing around 10 100 specimens; these specimens belong to 9 orders, 76 families, 234 genera and 334 species, of which 106 are endemics from Hispaniola Island. Of all registers, 68 % is identified at least to genera level, and around 3400 have been digitized, with Araneae representing more than 75 %. Most of the records are from Hispaniola, specifically from Dominican Republic, dating from 1974 to present; however, the collection also contains a few specimens from other Caribbean countries which were received through donations. The arachnids’ orders represented in the collection are: Acari (not included in the dataset), Amblypygi, Araneae, Opiliones, Pseudoscorpiones, Schizomida, Scorpiones, Solifugae, and Thelyphonida; with Palpigradi being the only one not represented of those recorded from Hispaniola.